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Kbiotech Quiz (July)


Rules and regulations

1

Number of codons for the amino acid Arginine is

(A) 4

(B) 6

(C) 1

(D) 2

2

Molecular Phylogenetic analysis suggests that Human are more closer to

(A) Orangutan

(B) Gorilla

(C) Chimpanzee

(D) Baboon

3

There are five different Histone Proteins in Eukaryotes. Which one of these is most conserved protein in the evolution?

(A) H1

(B) H2A

(C) H2B

(D) H3

4

80s Ribosome present in Eukaryotes. It is made up of two subunits 60s and 40s. 60s consists of 28s, 5.8s and 5s. All rRNA are coded by RNA Pol 1 accept

(A) 28s

(B) 5.8s

(C) 5s

(D) All rRNA are coded by RNA Pol 1

5

Reporter Gene are use to detect Transformed cells. The lux (Luciferase) gene is

(A) Scorable Reporter Gene

(B) Selectable Reporter Gene

(C) Both

(D) None

6

Same Plasmid can be present in three different topological forms. After Electrophoresis in gel the sequence of the bands will be

(A) Nicked > Linear > Supercoiled

(B) Linear > Nicked > Supercoiled

(C) Linear > Supercoiled > Nicked

(D) All will form single band because of same molecular weight

7

The Receptor through which HIV infects is

(A) CD2

(B) CD3

(C) CD4

(D) CD5

8

In a PCR experiment, a Researcher forgot to add one Primer. Both Primers (Forward and Reverse) are required for PCR amplification, both are different and both bind to the ends of the dsDNA. If there is only one dsDNA in the cocktail then how many times the DNA will amplify in the three complete cycles of PCR

(A) 2*2*2 = 8 Times

(B) 2*3 = 6 Times

(C) One Strand will amplify three times

(D) No amplification because both Primer are required for PCR amplification

9

Immunoglobulin deficiency can be detected by

(A) Flow Cytometry

(B) DTH skin test

(C) Mixed Lymphocyte Response (MLR)

(D) Serum Protein Electrophoresis

10

The Number of IITs (Indian Institute of Technology) at present is

(A) 5

(B) 7

(C) 11

(D) 12

 

Answer

 

B, C, D, C, A - A, C, C, D, B

 

1 (B) 6

Arginine, Leucine and Serine are coded by 6 codons

 

2 (C) Chimpanzee

 

3 (D) H3

H1 is the most variable, H2A and H2B varies in different species but H3 and H4 are most stable in evolution.

 

4 (C) 5s

RNA Polymerase III is synthesizes t RNA and 5 S rRNA

 

5 (A) Scorable Reporter Gene

Selectable Reporter gene product make capable to survive transformed cells under pressure.

 

6 (A) Nicked > Linear > Supercoiled

 

7 (C) CD4

 

8 (C) One Strand will amplify three times

 

9 (D) Serum Protein Electrophoresis

 

10 (B) 7

 

Winner

 

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