Biotechnology Notes
DNA modifying Enzymes Cutting and Joining DNA molecules, Genetic Engineering
Restriction endonuclease - RE 1, RE 2, Factors that Influence Restriction Enzyme Activity, Enzymes used in R-DNA technology, DNA Methyltransferases
Biology and Activity of Restriction Endonucleases, Cutting DNA with Restriction Endonucleases,
Joining DNA molecules - DNA Ligation
Nucleases: DNase and RNase, DNA Ligase, Alkaline Phosphatase, Polynucleotide Kinase, Alkaline Phosphatase
DNA Polymerases
E. coli DNA Polymerase I , Klenow Fragment of E. coli DNA Polymerase I
T4 DNA Polymerase , T7 DNA Polymerase
Thermostable DNA Polymerases (Taq, Pfu, Vent, etc.)
Adaptors, Linker, Homopolymer Tailing, Engineering DNA ends,
Plasmids and Vectors -
Plasmids, Plasmids, Plasmid copy number, Extrachromosomal Elements, Bacterial plasmids and DNA transformation,
Vectors, Vector Construction, Cloning vector and Expression vector
Prokaryotic Expression Vectors 1, PE vectors 2, High-capacity vectors
pBR322, pUC, Bacteriophage Lambda Vectors,
Bacteriophage M13, M13mp Vectors, λ phage, Cosmids, λ cosmids
P1 phage, Cosmids, BACs, PACs, Yeast Artificial Chromosomes (YACs)
Virulent bacteriophage methods
Gene Cloning
Basic cloning technique, Additional cloning methods, Cloning PCR Products, Cloning Genes,
Cloning Strategies
Libraries, M13 Phage Display Libraries
Cloning Genomic DNA - Genomic DNA Libraries, Gene Libraries
cDNA Cloning - cDNA, cDNA Libraries
Screening -
Sequencing
DNA sequencing, DNA Sequence Analysis, Sequencing Assembly, Genome Annotation
Mutagenesis
Gene Transfer
Transgenic plants, Somatic cell genetics and transgenics,
Genetic Engineering
Application, Combinatorial genetic engineering, Role of Microorganisms in Genetic Engineering
Recombinant DNA - re combining of two or more DNA molecules
rDNA, Selectable Markers & Markers for Screening
Gene function transmission (conjugation transformation transduction)
1) isolation identification of desired gene
2) insertion of desired gene in suitable Vector
3) insertion of this vector to suitable host/organism
4) selection of the transformed host cell
5) multiplication/expression of the desired gene
Molecular Markers
RAPD, RAPD's, Arbitrary-Primed PCR (Randomly Amplified Polymorphic DNA)
VNTR's, AFLP's, RFLP's, SSCP's, RAPD
Basic Techniques of Biotechnology
DNA Techniques -DNA Detection and Hybridization, DNA diagnostics, Primers
Nucleic acid blotting - Southerns, Northerns, Westerns, & Cloning Molecular Searching Techniques
Southern blotting, Northern blotting, Western blotting, Eastern blotting
Polymerase chain reaction - PCR and Thermalcycling, thermalcycler files
RT-PCR, Long accurate PCR (LA-PCR), Real-time quantitive PCR
Monoclonal Antibody Technology, Antibody methods
SELEX (Selective Evolution of Ligands by Exponential Enrichment)
Molecular Imprinting,
Gene Therapy
Nanotechnology and Microarray
Chips and microarrays, Microarray, Microarray II
Nucleic acids and polymerization
Finding the gene that encodes a purified protein, Temperate bacteriophage methods,
Potential Term Paper Topics for Biotech