Plant Biotech / Plant Tissue Culture Notes
Plant Tissue Culture Techniques
Importance of Plant Tissue Culture, Importance and Historical View of PTC
Culture Media, Culture Media2, Sterilization Techniques, Totipotency, media1, media2
Types of Cultures, Single Cell Cultures, Meristem Culture, Micropropagation, Micropropagation Methods, Anther/ Pollen Culture, Application of Haploids in Plant Breeding, Ovary/ Ovule Culture
Somaclonal Variations, Gametoclonal Variation
Plant Tissue Culture and Genetic Transformation
Plant Growth Regulators Used in Plant Tissue Culture, Hormones,
Full Hormones, Auxin, Cytokinin, Cytokinins, Cytokinins2, GA, Ethylene signaling, Bioassay for Gibberellin,
Auxin, Gibberllin, Abscisic Acid, Ethylene Gas, Ethylene signaling
Plant Growth Regulators Used in Plant Tissue Culture, Plant Growth Regulators, somatic embryogenesis
Protoplasts - Methods of Isolation, Culture and Regeneration of Protoplasts,
Protoplast Fusion and Somatic Hybridization
Transgenic Plants
Agrobacterium, Agrobacterium2, Gene transfer, Plant Transformation
Methods for production of transgenic plants / Gene delivery methods,
Plant vectors- Gene Manipulation with Agrobacterium Tumaefaciens,
Binary vectors, cointegration pTi and Ti plasmid
Viral gene transfer- Bromo mosaic virus (BMV), CaMV, Gemini virus, TMV
Direct Gene Transfer methods - microinjection, macroinjection
Direct DNA uptake, Lipofection, Electroporation, Particle gun method, Biolistic
by CaCl2 and PEG
Examples of Transgenic Plants, Transgenic Plants Applications, Adventitious regeneration
Herbicide resistance, pest resistant, drought resistant, salt resistant
Nitrogen fixation and biofertilizer, Nitrogen fixatation gene
Secondary Metabolite Production Alternative for Secondary Etabolite Production
Production of Secondary Metabolites
Biochemical Products of Interest - PART I- Pharmaceuticals
Biochemical Products of Interest - Part II- Other Products
Germplasm Preservation: Cryobiology
Biological control of pests and disease of crop plants, biopesticides
Dimensions of Plant Tissue Culture Research
Designing a Plant Micro propagation Laboratory
Plant Tissue Culture Terminology